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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Overexpression of the gene has long been noticed in lots of tumors, which results in chromosome instability, and so may well lead to tumorigenesis. This protein was discovered to affiliate with, and become associated with, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in mobile-cycle regulated histone gene expression and performs a important part in advertising mobile-cycle development from the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode unique isoforms, are already described. Two more splice variants had been claimed but specific nucleotide sequence details is not really nevertheless accessible.

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Blocking peptides are peptides that bind specially towards the focus on antibody and block antibody binding. These peptide ordinarily includes the epitope identified by the antibody.

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This information outlines further more doable optimization of cellular and immunoassay parameters to ensure the absolute best results are...

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This guide outlines further more achievable optimization of cellular and immunoassay parameters to make sure the absolute best benefits are...

Antibodies sure to the blocking peptide no more bind into the epitope over the concentrate on protein. By evaluating the staining from your blocked antibody compared to the antibody alone, you can see which staining is particular.

Target Information and facts Cyclin E belongs to your remarkably conserved cyclin loved ones, whose associates are characterized by a spectacular periodicity in protein abundance in the cell cycle. Cyclins purpose as regulators of CDK kinases. Distinct cyclins exhibit distinct expression and degradation patterns which contribute into the temporal coordination of each mitotic event. Cyclin E sorts a fancy with and capabilities like a regulatory subunit of CDK2, whose activity is needed for mobile cycle G1/S transition. This protein accumulates for the G1-S section boundary and it is degraded as cells development by means of thr77 S section.

The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both antibodies...

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